Mary Stuart or Stewart may refer to:
- Mary I of Scotland (1542–1587), also known as "Mary, Queen of Scots", wife of Francis II of France and mother of James I of England
- Mary Stuart, who died in 1607 at age two, infant daughter of James I of England
- Mary, Princess Royal and Princess of Orange (Mary Henrietta Stuart, 1631–1660), Princess Royal and Princess of Orange-Nassau. Daughter of Charles I of England and mother of William III of England
- Mary II of Scotland (1662–1694), co-ruler of England and Scotland with her husband William III from 1689 until her death
- Mary Stuart (actress) (1926–2002), American actress, most known for her 35-year role on Search for Tomorrow
- Mary Stuart Masterson, actress
- Maria Stuart (play), a tragic play by Friedrich Schiller based on the life of Mary I of Scotland
- Mary Stuart, a tragic opera by Gaetano Donizetti based on the play by Friedrich Schiller on the life of Mary I of Scotland
- Mary Stewart, Baroness Stewart of Alvechurch
- Mary Stuart, Countess of Bute
- Mary Stewart (swimmer)
- Mary Stewart, Duchess of Richmond (1622-1685)
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